Representation of genderfluid characters is limited. Although there is now much more representation of trans experience than previously existed, genderfluidity is still less commonly represented in media in comparison with other identities. However, there are some genderfluid characters, which are listed below. Each genderfluid person's experience is different, and some may find the characters' experiences to be vastly different from their own, while others may find it very relatable.

The majority of the genderfluid representation available is currently found in books. Some of the genderfluid characters in books are: Claude/Claudie from the book Baker Thief by Claudie Arseneault, Riley from the book Symptoms of Being Human by Jeff Garvin, Ax/Axel from the book Fluidity by E.M. Leya, Alex from the Magnus Chase series by Rick Riordan, Jerico/Jeri from the Arc of Scythe series by Neal Shusterman, Ash from the book Both Can Be True by Jules Machias, Brendan Chase from the book Freakboy by Kristin Elizabeth Clark, Sal from the Mask of Shadows duology by Linsey Miller, Aleks/Alexis from the book Somebody Told Me by Mia Siegert, Corey/Kori from the book Why We Fight by TJ Klune, Mars from The Honeys by Ryan Sala, Cielo from the The Brilliant Death series by Amy Rose Capetta, Harley from The Heartbreak Bakery by A.R. Capetta, and Clare from the book Under Shifting Stars by Alexanda Latos. Not all of these characters are labeled as "genderfluid" using that specific word (for instance, Aleks/Alexis only uses the term bigender), however they do change between genders and experience a fluidity of gender identity.

Genderfluid characters in shows include Val/Valentina from the animated series Gen:Lock and the demon Crowley from the TV show Good Omens.

Regarding movies, many consider the animated movie Nimona and titular shape-shifting character to be a depiction of genderfluidity and relate strongly with the character.

The webcomic Rain also includes a side character, Ky/Kylie, who is genderfluid.

As time goes on, more representation will continue to be created and depicted.